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| Registros recuperados: 27 | |
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| Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy. |
| Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)... |
| Tipo: Text |
Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00347/45828/45916.pdf |
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| Mauduit, Florian; Farrell, Anthony P.; Domenici, Paolo; Lacroix, Camille; Le Floch, Stephane; Lemaire, Pphilippe; Nicolas-kopec, Annabelle; Whittington, Mark; Le Bayon, Nicolas; Zambonino-infante, Jose-luis; Claireaux, Guy. |
| Consequences of exposure to polyaromatic hydrocarbons (PAH), toxic components of crude oil, on fish has been widely documented due to their ecological and economical importance. However, although forming a valuable and consistent body of knowledge, use of these data in spill response is limited. Objective of the present study was thus to facilitate the translation of published data into information of operational value. For this, we investigated the dose‐response relationship between dispersant‐treated oil exposure and ecologically‐relevant consequences by combining laboratory and field experiments. Effects were examined over almost a year using juveniles of the slow growing, commercially important European sea bass (Dicentrarchus labrax). Moreover, a... |
| Tipo: Text |
Palavras-chave: Oil spills; Polycyclic aromatic hydrocarbons (PAHs); Fish indices; Hypoxia; Temperature. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00455/56679/58438.pdf |
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| Cominassi, Louise; Moyano, Marta; Claireaux, Guy; Howald, Sarah; Mark, Felix C.; Zambonino-infante, Jose-luis; Le Bayon, Nicolas; Peck, Myron A.. |
| Ocean acidification and ocean warming (OAW) are simultaneously occurring and could pose ecological challenges to marine life, particularly early life stages of fish that, although they are internal calcifiers, may have poorly developed acid-base regulation. This study assessed the effect of projected OAW on key fitness traits (growth, development and swimming ability) in European sea bass (Dicentrarchus labrax) larvae and juveniles. Starting at 2 days post-hatch (dph), larvae were exposed to one of three levels of PCO2 (650, 1150, 1700 μatm; pH 8.0, 7.8, 7.6) at either a cold (15°C) or warm (20°C) temperature. Growth rate, development stage and critical swimming speed (Ucrit) were repeatedly measured as sea bass grew from 0.6 to ~10.0 (cold) or ~14.0... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00513/62473/66785.pdf |
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| Paul-pont, Ika; Tallec, Kevin; Gonzalez-fernandez, Carmen; Lambert, Christophe; Vincent, Dorothee; Mazurais, David; Zambonino-infante, Jose-luis; Brotons, Guillaume; Lagarde, Fabienne; Fabioux, Caroline; Soudant, Philippe; Huvet, Arnaud. |
| Marine plastic pollution is a major environmental issue. Given their ubiquitous nature and small dimensions, ingestion of microplastic (MP) and nanoplastic (NP) particles and their subsequent impact on marine life are a growing concern worldwide. Transfers along the trophic chain, including possible translocation, for which the hazards are less understood, are also a major preoccupation. Effects of MP ingestion have been studied on animals through laboratory exposure, showing impacts on feeding activity, reserve depletion and inflammatory responses, with consequences for fitness, notably reproduction. However, most experimental studies have used doses of manufactured virgin microspheres that may not be environmentally realistic. As for most... |
| Tipo: Text |
Palavras-chave: Microplastics; Nanoplastics; Experimental exposure; Impacts; Marine organisms; Physiology. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56088/57621.pdf |
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| Morais, Sophia; Cahu, Chantal; Zambonino-infante, Jose-luis; Robin, Jean; Ronnestad, I; Dinis, M; Conceicao, L. |
| The influence of dietary TAG source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mRNA level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. Fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. Coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain TAG. Growth was not related to lipase specific activity, suggesting a production in excess to dietary needs. Body lipid content was positively related to dietary lipid level and was affected by lipid quality. In addition, larval FA composition generally reflected that of the diet. The... |
| Tipo: Text |
Palavras-chave: Lipase activity; Dietary; Growth; Larvae; Sea bass; Fish culture. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-785.pdf |
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| Mahé, Kelig; Gourtay, Clemence; Bled--defruit, Geoffrey; Chantre, Celina; De Pontual, Helene; Amara, Rachid; Claireaux, Guy; Audet, C.; Zambonino-infante, Jose-luis; Ernande, Bruno. |
| Otolith shape is an efficient tool for fish stock discrimination. Therefore, it is crucial to identify the environmental parameters that could influence otolith morphogenesis and growth. Current global ocean warming negatively affects the production of polyunsaturated fatty acids (EPA and DHA) by phytoplankton and thus their availability in marine food webs. As EPA and DHA are essential nutrients for most fish species, their deficiency could affect many aspects of fish physiology, notably otolith morphogenesis. The goal of this study was to assess experimentally the combined influences of temperature (T) and dietary polyunsaturated fatty acid (PUFA) content on European seabass (Dicentrarchus labrax) otolith morphogenesis during early life stages. 300... |
| Tipo: Text |
Palavras-chave: N-3 polyunsaturated fatty acids; Otolith shape; Temperature effect; Elliptic Fourier descriptors; Multivariate mixed-effects models; Directional asymmetry. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/70054/68017.pdf |
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| Moyano, Marta; Candebat, Caroline; Ruhbaum, Yannick; Alvarez-fernandez, Santiago; Claireaux, Guy; Zambonino-infante, Jose-luis; Peck, Myron A.. |
| Most of the thermal tolerance studies on fish have been performed on juveniles and adults, whereas limited information is available for larvae, a stage which may have a particularly narrow range in tolerable temperatures. Moreover, previous studies on thermal limits for marine and freshwater fish larvae (53 studies reviewed here) applied a wide range of methodologies (e.g. the static or dynamic method, different exposure times), making it challenging to compare across taxa. We measured the Critical Thermal Maximum (CTmax) of Atlantic herring (Clupea harengus) and European seabass (Dicentrarchus labrax) larvae using the dynamic method (ramping assay) and assessed the effect of warming rate (0.5 to 9°C h-1) and acclimation temperature. The larvae of herring... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00395/50590/51282.pdf |
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| Vanderplancke, Gwenaelle; Claireaux, Guy; Quazuguel, Patrick; Huelvan, Christine; Corporeau, Charlotte; Mazurais, David; Zambonino-infante, Jose-luis. |
| Since European sea bass (Dicentrarchus labrax) larvae occurred in coastal and estuarine waters at early life stages, they are likely to be exposed to reduced dissolved oxygen waters at a sensitive developmental stage. However, the effects of hypoxia at larval stage, which depend in part on fish species, remain very poorly documented in European sea bass. In the present study, the impacts of an experimental exposure to a chronic moderate hypoxia (40 % air saturation) between 30 and 38 days post-hatching on the physiological and developmental traits of European sea bass larvae were assessed. This study was based on the investigation of survival and growth rates, parameters related to energy metabolism [Citrate Synthase (CS) and Cytochrome-c Oxidase (COX)... |
| Tipo: Text |
Palavras-chave: Hypoxia; Fish larvae; Energy metabolism; Maturation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00253/36433/35005.pdf |
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| Cominassi, Louise; Moyano, Marta; Claireaux, Guy; Howald, Sarah; Mark, Felix C.; Zambonino-infante, Jose-luis. |
| When organisms are unable to feed ad libitum they may be more susceptible to negative effects of environmental stressors such as ocean acidification and warming (OAW). We reared sea bass (Dicentrarchus labrax) at 15 or 20 °C and at ambient or high PCO2 (650 versus 1750 µatm PCO2; pH = 8.1 or 7.6) at ad libitum feeding and observed no discernible effect of PCO2 on the size-at-age of juveniles after 277 (20 °C) and 367 (15 °C) days. Feeding trials were then conducted including a restricted ration (25% ad libitum). At 15 °C, growth rate increased with ration but was unaffected by PCO2. At 20 °C, acidification and warming acted antagonistically and low feeding level enhanced PCO2 effects. Differences in growth were not merely a consequence of lower food intake... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00609/72074/70784.pdf |
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| Cadiz, L.; Desmarais, E.; Servili, Arianna; Quazuguel, Patrick; Madec, Lauriane; Huelvan, Christine; Andersen, O.; Zambonino-infante, Jose-luis; Mazurais, David. |
| Hemoglobins (Hb) play a critical role in satisfying the oxygen demand of vertebrate aerobic metabolism. The present study reports the characterization of the European sea bass (Dicentrarchus labrax) Hb genes, including genomic organization, phylogeny, and spatio-temporal gene expression. These Hb genes are divided into two unlinked clusters, the “MN” cluster containing eleven genes (five Hbα genes named MN-Hbα1-5 and six Hbβ genes named MN-Hbβ1–6) and the “LA” cluster consisting of three genes (two Hbα genes named LA-Hbα1-2 and one Hbβ gene named LA-Hbβ1). Comparative analysis of Hb amino acid sequences indicates that most of the important amino acid residues involved in hemoglobin-oxygen binding, particularly in the Bohr and Root effects, are generally... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00382/49349/49787.pdf |
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| Vanderplancke, Gwenaelle; Claireaux, Guy; Quazuguel, Patrick; Madec, Lauriane; Ferraresso, Serena; Severe, Armelle; Zambonino-infante, Jose-luis; Mazurais, David. |
| Hypoxia episodes have been generally studied over the last decade for their lethal consequences in coastal areas, which are nurseries for several fish species. The possible long-lasting effects of non-lethal hypoxia exposures at larval stage are, however, poorly documented. We investigated the long-lasting phenotypic impact of an experimental exposure to moderate hypoxia (40 % air saturation) between 30 and 38 days post-hatching in European sea bass (Dicentrarchus labrax). At the juvenile stage, our data indicate that this early hypoxia exposure impacted on juvenile growth rate but not on hypoxia tolerance. Growth depression was associated with a regulation of metabolism as suggested by a decrease in blood glycaemia, a decreased hepatic expression of the... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36517/36676.pdf |
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| Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R. |
| Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA... |
| Tipo: Text |
Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf |
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| Crespel, Amélie; Anttila, Katja; Lelièvre, Pernelle; Quazuguel, Patrick; Le Bayon, Nicolas; Zambonino-infante, Jose-luis; Chabot, Denis; Claireaux, Guy. |
| The accumulation of CO2 in the atmosphere and resulting ocean acidification represent a threat to marine ecosystems. While acid–base regulatory capacity is well developed in marine fish, allowing compensation of extra-cellular pH during short-term hypercapnia, the possible energetic costs of such regulation during long-term exposure remain to be established. In this study, juvenile European sea bass (Dicentrarchus labrax) were exposed from 2 days post-hatching to three different ocean acidification scenarios: control (present condition, PCO2 = 520 µatm, pH 7.9), moderate acidification (PCO2 = 950 µatm, pH 7.7), and high acidification (PCO2 = 1490 µatm, pH 7.5). After 1.5 years of exposure, fish aerobic metabolic capacities, as well as elements of their... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00512/62340/66604.pdf |
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| Cadiz, Laura; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Madec, Lauriane; Le Delliou, Herve; Mazurais, David. |
| Several physiological functions in fish are shaped by environmental stimuli received during early life. In particular, early-life hypoxia has been reported to have long-lasting effects on fish metabolism, with potential consequences for fish life history traits. In the present study, we examine whether the synergistic stressors hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), encountered during early life, could condition later metabolic response in European sea bass (Dicentrarchus labrax) juveniles. Growth rate and metabolic parameters related to carbohydrate and lipid metabolism in the liver were investigated at the juvenile stage under normoxic and chronic hypoxic conditions. Juvenile growth rates were significantly lower... |
| Tipo: Text |
Palavras-chave: Hypoxia; Developmental plasticity; Metabolism; European sea bass. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00404/51536/52126.pdf |
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| Cadiz, Laura; Ernande, Bruno; Quazuguel, Patrick; Servili, Arianna; Zambonino-infante, Jose-luis; Mazurais, David. |
| Environmental conditions, to which organisms are exposed during all their life, may cause possible adaptive responses with consequences in their subsequent life-history trajectory. The objective of this study was to investigate whether ecologically relevant combinations of hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), occurring during the larval period of European sea bass larvae (Dicentrarchus labrax), could have long-lasting impacts on the physiology of resulting juveniles. Hypoxic challenge tests were performed over one year to give an integrative evaluation of physiological performance. We revealed that juvenile performance was negatively impacted by hypoxia but not by the thermal conditions experienced at larval stage. This... |
| Tipo: Text |
Palavras-chave: Hypoxia; Temperature; Developmental plasticity; Carry-over effects; Opercular malformation; European sea bass. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00434/54606/55988.pdf |
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| Politis, Sebastian Nikitas; Sorensen, Sune R.; Mazurais, David; Servili, Arianna; Zambonino-infante, Jose-luis; Miest, Joanna J.; Clemmesen, Catriona M.; Tomkiewicz, Jonna; Butts, Ian A. E.. |
| Digestive system functionality of fish larvae relies on the onset of genetically pre-programmed and extrinsically influenced digestive functions. This study explored how algal supplementation (green-water) until 14 days post hatch (dph) and the ingestion of food [enriched rotifer (Brachionus plicatilis) paste] from 15 dph onward affects molecular maturation and functionality of European eel larval ingestion and digestion mechanisms. For this, we linked larval biometrics to expression of genes relating to appetite [ghrelin (ghrl), cholecystokinin (cck)], food intake [proopiomelanocortin (pomc)], digestion [trypsin (try), triglyceride lipase (tgl), amylase (amyl)], energy metabolism [ATP synthase F0 subunit 6 (atp6), cytochrome-c-oxidase 1 (cox1)], growth... |
| Tipo: Text |
Palavras-chave: Anguilla anguilla; Ingestion; Digestion; Gene expression; RNA/DNA; Aquaculture. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57445/59550.pdf |
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| Zambonino-infante, Jose-luis; Panserat, S.; Servili, Arianna; Mouchel, Olivier; Madec, Lauriane; Mazurais, David. |
| Optimizing the metabolic use of these carbohydrate-rich plant products in fish feeds could be achieved through nutritional programming strategies of fish larvae. The present study aims at testing the long-term effect of an early-life high-carbohydrate nutritional (34% of starch in the feed) stimulus in European sea bass (Dicentrarchus labrax), by examining several biochemical and molecular parameters involved in carbohydrate metabolism in sea bass larvae and juveniles, compared to a control group fed standard larval regime (phase 1). As awaited, specific activities of digestive enzymes, from pancreatic and intestinal segments, involved in glucide digestion (amylase, sucrase) were enhanced together with an up-regulation of glucokinase gene and... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Glucose metabolism; High-carbohydrate regimes; Marine fish larvae; Nutritional programming. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00470/58129/60552.pdf |
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| Vagner, Marie; Pante, Eric; Viricel, Amelia; Lacoue-labarthe, Thomas; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Dubillot, Emmanuel; Huet, Valerie; Le Delliou, Herve; Lefrançois, Christel; Imbert-auvray, Nathalie. |
| Highly unsaturated fatty acids of the omega-3 series (HUFA) are major constituents of cell membranes, yet poorly synthesised de novo by consumers. Their production, mainly supported by aquatic microalgae, has been decreasing with global change. Understanding the consequences of such reductions is essential for ectotherm consumers, since temperature tightly regulates the HUFA content in cell membranes, maintaining their functionality. Integrating individual, tissue and molecular approaches, we examined the consequences of the combined effects of temperature and HUFA depletion on the key cardio-respiratory functions of the golden grey mullet, an ectotherm grazer of high ecological importance. For four months, fish were exposed to two contrasting HUFA diets... |
| Tipo: Text |
Palavras-chave: Omega-3 highly unsaturated fatty acids; Temperature; Cardiac performance; Swimming performance; Chelon auratus; RNAseq. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00476/58744/63565.pdf |
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| Gatesoupe, Joel; Zambonino-infante, Jose-luis; Cahu, Chantal; Bergot, P. |
| Chez les poissons, le terme de larve désigne les plus jeunes individus entre le moment où ils sont capables d'ingérer de la nourriture exogène et celui où ils prennent la forme de l'adulte. Le stade larvaire suit le stade embryonnaire et précède le stade juvénile. II correspond à une période de transformation morphologique et physiologique continue de l'animal. La larve partage avec l'embryon certaines caractéristiques comme une très petite taille, un taux de croissance spécifique très élevé et une différenciation incomplète de certains organes. Cependant, elle est déjà capable de capturer et digérer des proies. |
| Tipo: Text |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00086/19713/17354.pdf |
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| Registros recuperados: 27 | |
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